Research areas: Pharmaceutical analysis, Analytical chemistry, Sample pretreatment, Mass spectrometry, Metabolomics and Proteomics


EDUCATION
2018-2019 Senior Postdoctoral Associate. Department of Biological Engineering,
Massachusetts Institute of Technology, USA
2015-2018 Postdoctoral Associate. Department of Biological Engineering,
Massachusetts Institute of Technology, USA
2010-2015 Ph.D. in Analytical Chemistry. College of Chemistry and Molecular Engineering,
Peking University, Beijing
2006-2010 Bachelor of Science in Chemistry. College of Chemistry and Molecular Sciences,
Wuhan University, Wuhan
RESEARCH EXPERIENCE
2015-2019 Department of Biological Engineering, Massachusetts Institute of Technology
Metabolomics study on a “human-on-a-chip” platform-supported by DARPA
Working in the DARPA “Human-On-A-Chip” projects mainly focus on the characterization and quantification of endogenous metabolic intermediates of tolcapone, investigation of related metabolic fate, as well as untargeted and targeted metabolomics for selected bio-samples.
Online capture of endogenous S-nitrosothiols (RSNO)
The aim of this research is to measure the endogenous RSNO in complicated real samples. Since RSNO is always in very low concentration, we have developed a phosphine-based chemical probe and applied it in automated solid phase derivatization (SPD), finally established an online SPD-LC-MS quantitation method for rapid and sensitive determination of RSNO.
Antibiotic lethality involves reactive nitrogen species (RNS)
Using quantitative biology, genetic and phenotypic approaches, we report a new killing mechanism mediated by bactericidal antibiotics in facultative anaerobic bacteria. Bactericidal antibiotics stimulate formation of reactive nitrogen species to contribute to antibiotic induced bacterial lethality.
2010-2015 College of Chemistry and Molecular Engineering, Peking University
University of California Berkeley USA Visiting Ph.D. Student
The major national scientific research project for plant hormones
Developing new labeling reagent and solid phase microextraction (SPME) coupled to LC-MS methods for plant hormones (brassinosteroids) analysis.
Exploring new sample pretreatment materials and establishing the online analytical methods
Synthesis the carbon nanotubes incorporated monolith and using it in SPME for the online coupling of SPME with direct analysis in real time mass spectrometry (DART-ToF-MS). The method was further expanded into the online coupling of SPME with PALDI-ToF-MS.
Developing novel chiral polymer materials for nano-LC separation
Preparation of the chiral MOFs incorporated monolith and its application in chiral separation by using the nano-LC system.
2006-2010 College of Chemistry and Molecular Sciences, Wuhan University
Preparation of polymer monolith and its application in SPME-LC-MS
National University Student Innovation Program-Novel organic light-emitting materials.
CO-WORK EXPERIENCE
2013-2014 Cooperation project with China Beverage Industry Association
Constructing models and developing GC-MS method for the analysis plasticizers in beverages.
2011-2012 Teaching assistant for experimental course
To guide undergraduate students to do experiments of GC-MS and CE.
2010-2012 Cooperation project with Agilent
As a third party, responsible for testing the performance of HPLC columns, including experimental design, data processing and evaluation of the results.
PROFILE SUMMARY
Pharmaceutical analytical chemistry, especially mass spectrometry-based biological and pharmaceutical analysis;
Targeted and untargeted metabolomic and proteomic studies;
Natural product research: extraction, separation and purification of compounds in natural products;
Development of sensitive derivatization LC-ESI-MS method for qualitative and quantitative analysis;
Exploring polymer monoliths for SPME, design and construction of automated online SPME-LC-MS methods;
Experienced with analytical methods and instruments (GC/LC-MS, CE-MS, DART-MS, PALDI-MS, Orbitrap);
Proficient in MassHunter/MassLynx, ChemStation, Element, Mass Profiler Professional (MPP), Endnote, Origin, Chemoffice, Photoshop, etc;
Capable of doing independent and collaborative research;
Reviewer for Journal of Separation Science, Chinese Journal of Chromatography, and RSC Advances;
English proficiency, proficient in communication and writing.
HONORS
First Place Prize for MIT-EHS Session, Massachusetts Institute of Technology, 2016.
Aoqing Tang Scholarship, Peking University, 2015.
Brilliance Scholarship, Peking University, 2014.
Yiliang Sun Anaytical Chemistry Scholarship, Peking University, 2013.
Prize for Exceptional Studies, Peking University, 2012.
Academic Scholarship, Peking University, 2011-2015.
First Prize for Innovation Competition, Hubei province, 2010.
Best Graduate Thesis Award, Hubei province, 2010.
Wuhan University Scholarship, Wuhan University, 2007, 2008, 2009.
PUBLICATIONS
Ahead of publication
[1] Xin Wang*, Clement T. Y. Chan, Dinelia Rivera-Burgos, Pete Wishnok, and Steven R. Tannenbaum*; Bactericidal antibiotics stimulate formation of reactive nitrogen species to contribute to antibiotic induced bacterial lethality; EMBO Reports, Under revision.
Already published: total citations for recent 5 years: 600, H-index: 12.
[1] Xin Wang*, Murat Cirit, John S. Wishnok, Linda G. Griffith, and Steven R. Tannenbaum*; Analysis of an Integrated Human Multi-organ Microphysiological System for Combined Tolcapone Metabolism and Brain Metabolomics; Analytical Chemistry, 2019, 91, 8667-8675. (Top 1 in Analytical Chemistry Field, JCR Q1, Impact Factor: 6.35)
[2] Xin Wang*, Carlos T. Garcia, Guanyu Gong, John S. Wishnok, and Steven R. Tannenbaum*; Automated Online Solid Phase Derivatization for Sensitive Quantification of Endogenous S-Nitrosoglutathione and Rapid Capture of Other Low-Molecular-Mass S-Nitrosothiols; Analytical Chemistry, 2018, 90, 1967-1975. (Top 1 in Analytical Chemistry Field, JCR Q1, Impact Factor: 6.35)
[3] Xin Wang, Xianjiang Li, Ze Li, Yiding Zhang, Yu Bai and Huwei Liu*; Online coupling of in-tube solid phase microextraction with direct analysis in real time mass spectrometry for rapid determination of triazine herbicides by using polymer monolith incorporated with single-wall carbon nanotubes; Analytical Chemistry, 2014, 86, 4739-4747. (Top 1 in Analytical Chemistry Field, JCR Q1, Impact Factor: 6.35, Highly Cited Paper: 180)
[4] Xin Wang, Xianjiang Li, Yu Bai and Huwei Liu*; Just dip it: Online coupling of “Dip-it” polymer monolith microextraction with plasma assisted laser desorption ionization mass spectrometry; Chemical Communications, 2015, 51, 4615-4618. (Top Journal, JCR Q1, Impact Factor: 6.29)
[5] Xin Wang, Qiao Ma, Min Li, Cuilan Chang, Yu Bai and Huwei Liu*; Automated and sensitive analysis of 28-epihomobrassinolide in Arabidopsis thaliana by on-line polymer monolith microextraction coupled to liquid chromatography-mass spectrometry; Journal of Chromatography A, 2013,1317, 121-128. (JCR Q1, Impact Factor: 3.86)
[6] Xin Wang, Alexandros Lamprou, Frantisek Svec,* and Huwei Liu*; Polymer-based Monolithic Column with Incorporated Chiral Metal-Organic Framework for Enantioseparation of Methyl Phenyl Sulfoxide Using Nano-Liquid Chromatography; Journal of Separation Science, 2016, 39, 4544-4548. (JCR Q2, Impact Factor: 2.51)
[7] Cuilan Chang, Xin Wang, Yu Bai, Huwei Liu*; Applications of nanomaterials in enantioseparation and related techniques; Trends in Analytical Chemistry, 2012, 39, 195-206. (Top Journal, JCR Q1, Impact Factor: 8.49)
[8] Feifeng Huo, Xin Wang, Yehua Han, Yu Bai, Wei Zhang, Hancheng Yuan, Huwei Liu*; A new derivatization approach for the rapid and sensitive analysis of brassinosteroids by using ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry; Talanta, 2012, 99, 420-425. (JCR Q1, Impact Factor: 4.91)
[9] Xianjiang Li, Xin Wang, Wen Ma, Wanpeng Ai, Li Ding and Huwei Liu*; Fast analysis of glycosides based on HKUST-1-coated monolith solid-phase microextraction and direct analysis in real-time mass spectrometry; Journal of Separation Science, 2017, 40, 1589-1596. (JCR Q2, Impact Factor: 2.51)
[10] Qiongwei Yu, Xin Wang, Qiao Ma, Bifeng Yuan, Haibo He and Yuqi Feng*; Automated analysis of non-steroidal anti-inflammatory drugs in human plasma and water samples by in-tube solid-phase microextraction coupled to liquid chromatography-mass spectrometry based on a poly(4-vinylpyridine-co-ethylene dimethacrylate) monolith; Analytical Methods, 2011, 4, 1538-1545.
[11] Xianjiang Li, Xin Wang, Linnan Li, Yu Bai and Huwei Liu*; Direct Analysis in Real Time Mass Spectrometry: a Powerful Tool for Fast Analysis; Mass Spectrometry Letters, 2015, 6, 1-6.
[12] Xianjiang Li, Ze Li, Xin Wang, Honggang Nie, Yiding Zhang, Yu Bai and Huwei Liu*; Monolith dip-it: a bifunctional device for improving the sensitivity of direct analysis in real time mass spectrometry; Analyst, 2016, 141, 4947-4952.
[13] Xianjiang Li, Cuilan Chang, Xin Wang, Yu Bai and Huwei Liu*; Application of homochiral metal-organic frameworks in enantioselective adsorption and chromatography separation; Electrophoresis, 2014, 35, 2733-2743.
[14] Xianjiang Li, Jiawei Xing, Cuilan Chang, Xin Wang, Yu Bai, Xiuping Yan and Huwei Liu*; Solid-phase extraction with the metal–organic framework MIL-101(Cr) combined with direct analysis in real time mass spectrometry for the fast analysis of triazine herbicides; Journal of Separation Science, 2014, 37, 1489-1495.
[15] Cuilan Chang, Xiaoyue Qi, Jiangwei Zhang, Yaming Qiu, Xianjiang Li, Xin Wang, Yu Bai, Jun-Liang Sun and Huwei Liu*; Facile synthesis of magnetic homochiral metal-organic frameworks for “enantioselective fishing”; Chemical Communications, 2015, 51, 3566-3569.
[16] Haitham Amal, Boaz Barak, Vadiraja Bhat, Guanyu Gong, Brian A. Joughin, Xin Wang, John S. Wishnok, Guoping Feng, Steven R. Tannenbaum*; Shank3 mutation in a mouse model of autism leads to changes in the S-nitroso-proteome and affects key proteins involved in vesicle release and synaptic function; Molecular Psychiatry, 2018, 1, 1-14. (Nature series)
[17] Haitham Amal*, Guanyu Gong, Hongmei Yang, Brian A. Joughin, Xin Wang, Charles G. Knutson, Maryam Kartawy, Igor Khaliulin, John S. Wishnok, Steven R. Tannenbaum*; Low doses of arsenic in a mouse model of human exposure and in neuronal culture lead to S-Nitrosylation of synaptic proteins and apoptosis via nitric oxide; International Journal of Molecular Sciences, 2020, 21, 3948-3967.